benzidine test

benzidine test n a test for blood (as in feces) based on its production of a blue color in a solution of benzidine, sodium peroxide, and glacial acetic acid

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(for occult blood in urine or feces) benzidine, acetic acid, and hydrogen peroxide are added to the specimen; hemoglobin catalyzes the oxidation of benzidine by hydrogen peroxide, giving a blue color. This is the most sensitive screening test for occult blood, but it is seldom used because benzidine is a carcinogen, and its use is restricted.

Medical dictionary. 2011.

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