affinity chromatography

affinity chromatography n chromatography in which a macromolecule (as a protein) is isolated and purified by passing it in solution through a column that has been treated with a substance having a ligand for which the macromolecule has an affinity that causes it to be retained on the column

* * *

chromatography based on a highly specific biologic interaction such as that between antigen and antibody, enzyme and substrate, or receptor and ligand. Any of these substances, covalently linked to an insoluble support or immobilized in a gel, may serve as the sorbent allowing the interacting substance to be isolated from relatively impure samples; often a 1000-fold purification can be achieved in one step.

Medical dictionary. 2011.

Look at other dictionaries:

  • Affinity chromatography — is a method of separating biochemical mixtures and based on a highly specific interaction such as that between antigen and antibody, enzyme and substrate, or receptor and ligand. Contents 1 Uses 2 Principle 3 Batch and column setup …   Wikipedia

  • affinity chromatography — A method for purifying specific components in a solution by exploiting their specific binding to known molecule(s). The mixed solution is passed through a column containing a solid medium to which the binding molecule is covalently attached. See …   Glossary of Biotechnology

  • affinity chromatography — giminiškumo chromatografija statusas T sritis Standartizacija ir metrologija apibrėžtis Chromatografija, pagrįsta biologiškai aktyvių medžiagų sąveika su gėriklio ligandais. atitikmenys: angl. affinity chromatography; biospecific affinity… …   Penkiakalbis aiškinamasis metrologijos terminų žodynas

  • affinity chromatography — giminingumo chromatografija statusas T sritis chemija apibrėžtis Chromatografija, pagrįsta biologiškai aktyvių medžiagų sąveika su sorbento ligandais. atitikmenys: angl. affinity chromatography; biospecific chromatography rus. афинная… …   Chemijos terminų aiškinamasis žodynas

  • affinity chromatography — Chromatography in which the immobile phase (bed material) has a specific biological affinity for the substance to be separated or isolated, such as the affinity of an antibody for its antigen, or an enzyme for a substrate analogue …   Dictionary of molecular biology

  • affinity chromatography — noun Date: 1970 chromatography in which a macromolecule (as a protein) is isolated and purified by passing it in solution through a column treated with a substance having a ligand for which the macromolecule has an affinity that causes it to be… …   New Collegiate Dictionary

  • affinity chromatography — noun : chromatography in which a macromolecule (as a protein) is isolated and purified by passing it in solution through a column that has been treated with a substance having a ligand for which the macromolecule has an affinity that causes it to …   Useful english dictionary

  • biospecific affinity chromatography — giminiškumo chromatografija statusas T sritis Standartizacija ir metrologija apibrėžtis Chromatografija, pagrįsta biologiškai aktyvių medžiagų sąveika su gėriklio ligandais. atitikmenys: angl. affinity chromatography; biospecific affinity… …   Penkiakalbis aiškinamasis metrologijos terminų žodynas

  • metal affinity chromatography — A chromatographic technique, in which a compound interacting with a specific metal ion can be captured by immobilizing the relevant ion on the column s solid matrix …   Glossary of Biotechnology

  • pseudo-affinity chromatography — A chromatographic technique in which a ligand is immobilized selectively to retain enzymes or other proteins …   Glossary of Biotechnology

Share the article and excerpts

Direct link
Do a right-click on the link above
and select “Copy Link”

We are using cookies for the best presentation of our site. Continuing to use this site, you agree with this.