immunofluorescence


immunofluorescence
An immunohistochemical technique using labeling of antibodies by a fluorescent dye to identify antigenic material specific for the labeled antibody; the specific binding of antibody can be determined microscopically through the production of a characteristic visible light by the application of ultraviolet rays to the preparation. SEE ALSO: fluorescent antibody technique.
- direct i. fluorescence microscopy of tissue from lesions after application of labeled antibodies. SEE ALSO: fluorescent antibody technique.
- indirect i. fluorescence microscopy of normal tissue after application of the patient's serum, to detect antibodies to normal tissue components (autoantibodies). SEE ALSO: fluorescent antibody technique.

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im·mu·no·flu·o·res·cence -(.)flu̇(-ə)r-'es-ən(t)s, -flōr-, -flȯr- n the labeling of antibodies or antigens with fluorescent dyes esp. for the purpose of demonstrating the presence of a particular antigen or antibody in a tissue preparation or smear
im·mu·no·flu·o·res·cent -ənt adj

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n.
a technique for observing the amount and/or distribution of antibody or antigen in a tissue section. The antibodies are labelled (directly or indirectly) with a fluorescent dye (e.g. fluorescein) and applied to the tissue, which is observed through an ultraviolet microscope. In direct immunofluorescence the antibody is labelled before being applied to the tissue. In indirect immunofluorescence the antibody is labelled after it has bound to the antigen, by means of fluorescein-labelled anti-immunoglobulin serum.
immunofluorescent adj.

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im·mu·no·flu·o·res·cence (im″u-no-fl-resґəns) any immunohistochemical method that uses antibody labeled with a fluorescent dye.

Immunofluorescence (green) used to detect pemphigus vulgaris.


Medical dictionary. 2011.

Look at other dictionaries:

  • Immunofluorescence — is the labeling of antibodies or antigens with fluorescent dyes. This technique is often used to visualize the subcellular distribution of biomolecules of interest. Immunofluorescent labelled tissue sections or cultures are studied using a… …   Wikipedia

  • immunofluorescence — [ imynoflyɔresɑ̃s ] n. f. • 1965; de immuno et fluorescence ♦ Biol. Technique utilisant un marquage fluorescent d anticorps spécifiques afin de visualiser et de localiser les antigènes correspondants. ● immunofluorescence nom féminin Technique de …   Encyclopédie Universelle

  • immunofluorescence — [im΄yə nōflô res′əns, i myo͞o΄nōflô res′əns] n. the use of a special microscope with ultraviolet light and antibodies labeled with fluorescent dyes to locate specific antigens in tissues immunofluorescent adj …   English World dictionary

  • Immunofluorescence — L’immunofluorescence est une technique d’immunomarquage, qui utilise des anticorps (ou immunoglobulines) ainsi que des fluorochromes. Sommaire 1 Historique 2 Principes de l immunofluorescence[1],[2],[3]. 2.1 Immunofluorescence …   Wikipédia en Français

  • immunofluorescence — n. a technique for observing the amount and/or distribution of antibody or antigen in a tissue section. The antibodies are labelled (directly or indirectly) with a fluorescent dye (e.g. fluorescein) and applied to the tissue, which is observed… …   The new mediacal dictionary

  • immunofluorescence — noun Date: 1960 the labeling of antibodies or antigens with fluorescent dyes especially for the purpose of demonstrating the presence of a particular antigen or antibody in a tissue preparation or smear • immunofluorescent adjective …   New Collegiate Dictionary

  • immunofluorescence — (im u no floo o res ens) A technique used to identify particular antigens microscopically in cells or tissues by the binding of a fluorescent antibody conjugate …   Dictionary of microbiology

  • immunofluorescence — A test or technique in which one or other component of an immunological reaction is made fluorescent by coupling with a fluorochrome such as fluorescein, phycoerythrin or rhodamine so that the occurrence of the reaction can be detected as a… …   Dictionary of molecular biology

  • immunofluorescence — immunofluorescent, adj. /im yeuh noh floo res euhns, flaw , floh , i myooh /, n. any of various techniques for detecting an antigen or antibody in a sample by coupling its specifically interactive antibody or antigen to a fluorescent compound,… …   Universalium

  • immunofluorescence — noun a technique that uses a fluorochrome to indicate a specific antigen antibody reaction …   Wiktionary


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