nitrate reduction test

(for bacteriuria or presence of bacteria in other fluids) a type of nitrite test that identifies the reduction of nitrate to nitrite by a bacterial culture. The fluid under investigation is cultured in a broth containing nitrate and the medium is tested for nitrite by mixing with solutions containing sulfanilic acid and alpha-naphthylamine in 5 N acetic acid; a red color indicates the presence of nitrite. The test is useful in identifying doubtful strains of Enterobacteriaceae, mycobacteria, and certain aerobic bacteria.

Medical dictionary. 2011.

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