polyacrylamide gel electrophoresis

(PAGE) gel electrophoresis using a polymerized polyacrylamide matrix to separate molecules on the basis of size, charge, or both; usually used to separate proteins or sequence nucleic acids. Gels are usually discontinuous (see disc e.) but may be a single layer, and are either nondenaturing to examine native molecules or denaturing as in SDS-PAGE.

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  • Gel electrophoresis — apparatus – An agarose gel is placed in this buffer filled box and electrical field is applied via the power supply to the rear. The negative terminal is at the far end (black wire), so DNA migrates toward the camera. Classification… …   Wikipedia

  • Gel electrophoresis — electrophoresis electrophoresis n. 1. (Chem.) the motion of charged molecules or particles in a liquid medium under the influence of an electric field; particles with a positive charge move toward the cathode and negative to the anode. [WordNet… …   The Collaborative International Dictionary of English

  • gel electrophoresis — noun Date: 1955 electrophoresis in which molecules (as proteins and nucleic acids) migrate through a gel and especially a polyacrylamide gel and separate into bands according to size …   New Collegiate Dictionary

  • Agarose gel electrophoresis — is a method used in biochemistry and molecular biology to separate DNA, or RNA molecules by size. This is achieved by moving negatively charged nucleic acid molecules through an agarose matrix with an electric field (electrophoresis). Shorter… …   Wikipedia

  • Two-dimensional gel electrophoresis — Two dimensional gel electrophoresis, abbreviated as 2 DE or 2 D electrophoresis, is a form of gel electrophoresis commonly used to analyze proteins. Mixtures of proteins are separated by two properties in two dimensions on 2D gels.Basis for… …   Wikipedia

  • electrophoresis — n. 1. (Chem.) the motion of charged molecules or particles in a liquid medium under the influence of an electric field; particles with a positive charge move toward the cathode and negative to the anode. [WordNet sense 1] Syn: cataphoresis.… …   The Collaborative International Dictionary of English

  • Polyacrylamide — (IUPAC poly(2 propenamide) or poly(1 carbamoylethylene)) is a polymer ( CH2CHCONH2 ) formed from acrylamide subunits that can also be readily cross linked. Acrylamide needs to be handled using good laboratory practices (GLP) to avoid poisonous… …   Wikipedia

  • DNA electrophoresis — is an analytical technique used to separate DNA fragments by size. An electric field forces the fragments to migrate through a gel. DNA molecules normally migrate from negative to positive potential due to the net negative charge of the phosphate …   Wikipedia

  • SDS-PAGE — PAGE redirects here. For other uses, see Page (disambiguation). Picture of an SDS PAGE. The molecular marker is in the left lane SDS PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis, describes a collection of related techniques… …   Wikipedia

  • Western blot — The western blot (alternatively, immunoblot) is an analytical technique used to detect specific proteins in a given sample of tissue homogenate or extract. It uses gel electrophoresis to separate native or denatured proteins by the length of the… …   Wikipedia

  • Molecular biology — (pronounced /məˈlɛkjʊlər .../) is the branch of biology that deals with the molecular basis of biological activity. This field overlaps with other areas of biology and chemistry, particularly genetics and biochemistry. Molecular biology… …   Wikipedia


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